Relationship between post-cardiac arrest myocardial oxidative stress and myocardial dysfunction in the rat

LI VOLTI, Giovanni;SORRENTI, Valeria;
2014-01-01

2014
BackgroundReperfusion after resuscitation from cardiac arrest (CA) is an event that increases reactiveoxygen species production leading to oxidative stress. More specifically, myocardialoxidative stress may play a role in the severity of post-CA myocardial dysfunction. Thisstudy investigated the relationship between myocardial oxidative stress and post-CAmyocardial injury and dysfunction in a rat model of CA and cardiopulmonary resuscitation(CPR). Ventricular fibrillation was induced in 26 rats and was untreated for 6 min. CPR,including mechanical chest compression, ventilation, and epinephrine, was then initiated andcontinued for additional 6 min prior to defibrillations. Resuscitated animals were sacrificed attwo h (n = 9), 4 h (n = 6) and 72 h (n = 8) following resuscitation, and plasma collected forassessment of: high sensitivity cardiac troponin T (hs-cTnT), as marker of myocardial injury;isoprostanes (IsoP), as marker of lipid peroxidation; and 8-hydroxyguanosine (8-OHG), asmarker of DNA oxidative damage. Hearts were also harvested for measurement of tissue IsoPand 8-OHG. Myocardial function was assessed by echocardiography at the correspondingtime points. Additional 8 rats were not subjected to CA and served as baseline controls.ResultsCompared to baseline, left ventricular ejection fraction (LVEF) was reduced at 2 and 4 hfollowing resuscitation (p < 0.01), while it was similar at 72 h. Inversely, plasma hs-cTnTincreased, compared to baseline, at 2 and 4 h post-CA (p < 0.01), and then recovered at 72 h.Similarly, plasma and myocardial tissue IsoP and 8-OHG levels increased at 2 and 4 h postresuscitation(p < 0.01 vs. baseline), while returned to baseline 72 h later. Myocardial IsoPwere directly related to hs-cTnT levels (r = 0.760, p < 0.01) and inversely related to LVEF (r= -0.770, p < 0.01). Myocardial 8-OHG were also directly related to hs-cTnT levels (r =0.409, p < 0.05) and inversely related to LVEF (r = -0.548, p < 0.01).ConclusionsThe present study provides evidence that lipid peroxidation and DNA oxidative damage inmyocardial tissue are closely related to myocardial injury and LV dysfunction during theinitial hours following CA.
Cardiopulmonary resuscitation,; Myocardial injury,; Oxidative damage,
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/42824
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