Grapevine yellows (GY) symptoms on grapevine cultivars Chardonnay and Inzolia have been reported in Sicily since many years. In some cases the molecular analyses allowed to identify in infected tissues phytoplasmas belonging to 16SrI-G genetic subgroup (today 16Sr-XII-Stolbur group) but never the Flavescence dorée (FD)-associated phytoplasma. The only FD-vector, the leafhopper Scaphoideus titanus, was never found. In the present work the results of surveys carried out in collaboration with the Regional Phytosanitary Service and in the framework of a three years Italian research project of the Italian Ministry of Agriculture are reported. GY symptoms were observed on different grapevine cultivars grown in the main Sicilian vineyard areas, either allochthonous or autochthonous. Direct and nested polymerase chain reaction (PCR) analyses allowed to identify phytoplasmas belonging to the 16SrXII-A-Stolbur group (LN) and to confirm the absence of the FD-associated phytoplasma.
In Sicilia sintomi di giallumi della vite (GY) sono stati segnalati, già da diversi anni, in viti delle cv Chardonnay ed Inzolia; in alcuni casi le analisi molecolari hanno consentito di individuare fitoplasmi appartenenti al sottogruppo genetico 16SrI-G (oggi gruppo 16SrXII-Stolbur, Albanese et al., 1996) ma non è mai stato riscontrato il fitoplasma associato a Flavescenza dorata (FD) né il suo unico vettore Scaphoideus titanus.Nella presente nota si riportano i risultati delle indagini, avviate in collaborazione con il Servizio Fitosanitario Regionale e nell’ambito di un progetto di ricerca triennale nazionale del MiPAF, mirate ad approfondire le conoscenze sull’eziologia e la distribuzione nel territorio dei GY in Sicilia. Sono stati riscontrati sintomi di GY in diverse cv, sia alloctone che autoctone, allevate nelle principali zone vitivinicole siciliane; le analisi di amplificazione genica diretta (PCR) e ripetuta (nested-PCR) hanno consentito di rilevare fitoplasmi del sottogruppo 16rXIIA Stolbur (LN) e di confermare l’assenza del fitoplasma associato a FD
Indagine sui giallumi della vite in Sicilia
LA ROSA, Rosa;TESSITORI, MATILDE;
2006-01-01
Abstract
Grapevine yellows (GY) symptoms on grapevine cultivars Chardonnay and Inzolia have been reported in Sicily since many years. In some cases the molecular analyses allowed to identify in infected tissues phytoplasmas belonging to 16SrI-G genetic subgroup (today 16Sr-XII-Stolbur group) but never the Flavescence dorée (FD)-associated phytoplasma. The only FD-vector, the leafhopper Scaphoideus titanus, was never found. In the present work the results of surveys carried out in collaboration with the Regional Phytosanitary Service and in the framework of a three years Italian research project of the Italian Ministry of Agriculture are reported. GY symptoms were observed on different grapevine cultivars grown in the main Sicilian vineyard areas, either allochthonous or autochthonous. Direct and nested polymerase chain reaction (PCR) analyses allowed to identify phytoplasmas belonging to the 16SrXII-A-Stolbur group (LN) and to confirm the absence of the FD-associated phytoplasma.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
