As a ®rst step towards using cross-species comparison to complete the inventory of the nuclear genes that encode mitochondrial polypeptides, and ul- timately to understand their function through systematic molecular and genetic analysis in a model organism of choice, we report here the characterization of 41 Dros- ophila melanogaster cDNAs. These cDNAs were isolated by screening an ovarian expression library with anti- bodies against mitochondrial proteins and identify 17 novel Drosophila genes. The deduced amino acid se- quences encoded by the majority of these cDNAs turned out to show signi®cant homology to mitochondrial proteins previously identi®ed in other species. Among others, ORFs putatively encoding six dierent subunits of ATP synthase and three NADH:ubiquinone reduc- tase subunits were detected. By in situ hybridization, all cDNAs were mapped to single bands on polytene chromosomes, thus identifying candidate Drosophila genes required for mitochondrial biogenesis and main- tenance. A search of the Human Gene Index database made it possible in most cases to align the entire Dros- ophila coding sequence with a human consensus se- quence, suggesting that the cDNAs originate from insect counterparts of expressed mammalian genes. Our ex- perimental strategy represents an ecient approach to the identi®cation and interspecies comparison of genes encoding products targeted to the mitochondrion.
|Titolo:||A strategy for the identification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database|
|Data di pubblicazione:||1999|
|Appare nelle tipologie:||1.1 Articolo in rivista|