The tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae), is a majorinvasive pest that has spread throughout many countries in the Mediterraneanbasin and parts of Asia over the last decade. The control of T. absolutahas relied heavily on the use of chemical insecticides, a strategy thathas led to the evolution of resistance. In this study, biological and molecularmethods were used to determine the susceptibility of five strains ofT. absoluta to the organophosphate chlorpyrifos and to investigate themolecular mechanisms underlying resistance to this class of insecticides.High levels of resistance to chlorpyrifos were observed in all five strainstested. Cloning and sequencing of the gene encoding the organophosphatetarget site, ace-1, of T. absoluta revealed the presence of an alanineto serine substitution at a position that has been previously linked withorganophosphate resistance across a range of different insect and mite species.The presence of this mutation at high frequency in T. absoluta populationsoriginating from various countries further supports the suggestionthat the rapid expansion of this species is, in part, mediated by the resistanceof this pest to chemical insecticides.

Mutation in the ace-1 gene of the tomato leaf miner (Tuta absoluta) associated with organophosphates resistance

RAPISARDA, Carmelo;
2017-01-01

Abstract

The tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae), is a majorinvasive pest that has spread throughout many countries in the Mediterraneanbasin and parts of Asia over the last decade. The control of T. absolutahas relied heavily on the use of chemical insecticides, a strategy thathas led to the evolution of resistance. In this study, biological and molecularmethods were used to determine the susceptibility of five strains ofT. absoluta to the organophosphate chlorpyrifos and to investigate themolecular mechanisms underlying resistance to this class of insecticides.High levels of resistance to chlorpyrifos were observed in all five strainstested. Cloning and sequencing of the gene encoding the organophosphatetarget site, ace-1, of T. absoluta revealed the presence of an alanineto serine substitution at a position that has been previously linked withorganophosphate resistance across a range of different insect and mite species.The presence of this mutation at high frequency in T. absoluta populationsoriginating from various countries further supports the suggestionthat the rapid expansion of this species is, in part, mediated by the resistanceof this pest to chemical insecticides.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/46642
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