A rapid high-performance liquid chromatographic method using a C18 reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO4·7H2O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH3CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H3PO4. Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays
Rapid high-performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor
BUCOLO, CLAUDIO
2002-01-01
Abstract
A rapid high-performance liquid chromatographic method using a C18 reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO4·7H2O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH3CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H3PO4. Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays| File | Dimensione | Formato | |
|---|---|---|---|
|
Rapid high-performance liquid chromatographic assay.pdf
solo gestori archivio
Tipologia:
Versione Editoriale (PDF)
Dimensione
52.51 kB
Formato
Adobe PDF
|
52.51 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
