A rapid high-performance liquid chromatographic method using a C18 reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO4·7H2O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH3CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H3PO4. Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays

Rapid high-performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor

BUCOLO, CLAUDIO
2002-01-01

Abstract

A rapid high-performance liquid chromatographic method using a C18 reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO4·7H2O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH3CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H3PO4. Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/4901
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