Osteoarthritis (OA) of the knee is a major problem in our society. The development of new treatmentoptions for OA is limited, because the pathophysiological mechanisms are not clearly understood, especiallyon the molecular level. Aquaporin 1 (AQP1) is a specific protein channels for water transport; itis expressed in articular chondrocytes, human synovitis, in chondrocytes of patients with rheumatoidarthritis or OA and in chondrocyte-like cells of human intervertebral disc. The aim of this study was toinvestigate the expression of AQP1, through immunohistochemistry, immunocytochemistry and Westernblot, in experimentally induced OA knee menisci. AQP1 was studied in vivo in knee OA menisci from36 rats that underwent medial or lateral meniscectomy, and in vitro on fibrochondrocytes derived fromknee OA menisci rats. OA in rats was experimentally induced and tested by histomorphometric analysis.Histological results demonstrated structural alterations in OA menisci accompanied by a very strongAQP1 immunohistochemical and immunocytochemical staining. The Western blot analysis confirmed astrong expression of AQP1 in OA fibrochondrocytes cells. The results of the present research suggest thatan activation of AQP1, induced by the OA process, may represent an endogenous mechanism, which canbe used to control the tissue degeneration within OA articular joints.

Osteoarthritis (OA) of the knee is a major problem in our society. The development of new treatment options for OA is limited, because the pathophysiological mechanisms are not clearly understood, especially on the molecular level. Aquaporin 1 (AQP1) is a specific protein channels for water transport; it is expressed in articular chondrocytes, human synovitis, in chondrocytes of patients with rheumatoid arthritis or OA and in chondrocyte-like cells of human intervertebral disc. The aim of this study was to investigate the expression of AQP1, through immunohistochemistry, immunocytochemistry and Western blot, in experimentally induced OA knee menisci. AQP1 was studied in vivo in knee OA menisci from 36 rats that underwent medial or lateral meniscectomy, and in vitro on fibrochondrocytes derived from knee OA menisci rats. OA in rats was experimentally induced and tested by histomorphometric analysis. Histological results demonstrated structural alterations in OA menisci accompanied by a very strong AQP1 immunohistochemical and immunocytochemical staining. The Western blot analysis confirmed a strong expression of AQP1 in OA fibrochondrocytes cells. The results of the present research suggest that an activation of AQP1, induced by the OA process, may represent an endogenous mechanism, which can be used to control the tissue degeneration within OA articular joints.

Aquaporin 1 (AQP1) expression in experimentally induced osteoarthritic knee menisci: an in vivo and in vitro study

MUSUMECI, GIUSEPPE
Primo
;
LEONARDI, Rosalia Maria;CARDILE, Venera;LORETO, CARLA AGATA
Ultimo
2013

Abstract

Osteoarthritis (OA) of the knee is a major problem in our society. The development of new treatment options for OA is limited, because the pathophysiological mechanisms are not clearly understood, especially on the molecular level. Aquaporin 1 (AQP1) is a specific protein channels for water transport; it is expressed in articular chondrocytes, human synovitis, in chondrocytes of patients with rheumatoid arthritis or OA and in chondrocyte-like cells of human intervertebral disc. The aim of this study was to investigate the expression of AQP1, through immunohistochemistry, immunocytochemistry and Western blot, in experimentally induced OA knee menisci. AQP1 was studied in vivo in knee OA menisci from 36 rats that underwent medial or lateral meniscectomy, and in vitro on fibrochondrocytes derived from knee OA menisci rats. OA in rats was experimentally induced and tested by histomorphometric analysis. Histological results demonstrated structural alterations in OA menisci accompanied by a very strong AQP1 immunohistochemical and immunocytochemical staining. The Western blot analysis confirmed a strong expression of AQP1 in OA fibrochondrocytes cells. The results of the present research suggest that an activation of AQP1, induced by the OA process, may represent an endogenous mechanism, which can be used to control the tissue degeneration within OA articular joints.
Osteoarthritis (OA) of the knee is a major problem in our society. The development of new treatmentoptions for OA is limited, because the pathophysiological mechanisms are not clearly understood, especiallyon the molecular level. Aquaporin 1 (AQP1) is a specific protein channels for water transport; itis expressed in articular chondrocytes, human synovitis, in chondrocytes of patients with rheumatoidarthritis or OA and in chondrocyte-like cells of human intervertebral disc. The aim of this study was toinvestigate the expression of AQP1, through immunohistochemistry, immunocytochemistry and Westernblot, in experimentally induced OA knee menisci. AQP1 was studied in vivo in knee OA menisci from36 rats that underwent medial or lateral meniscectomy, and in vitro on fibrochondrocytes derived fromknee OA menisci rats. OA in rats was experimentally induced and tested by histomorphometric analysis.Histological results demonstrated structural alterations in OA menisci accompanied by a very strongAQP1 immunohistochemical and immunocytochemical staining. The Western blot analysis confirmed astrong expression of AQP1 in OA fibrochondrocytes cells. The results of the present research suggest thatan activation of AQP1, induced by the OA process, may represent an endogenous mechanism, which canbe used to control the tissue degeneration within OA articular joints.
knee menisci; Aquaporin 1; osteoarthritic
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/50498
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