We investigated the relation between apoptosis and delayed luminescence (DL) in human leukemia Jurkat Tcells undergoing various treatments. We used menadione, hydrogen peroxide and quercetin to induce oxidative stress conditions under different doses and treatment times. We irradiated Jurkat cells by using modulated beams of accelerated protons with energies up to 62 MeV, under a dose of 10 Gy distributed uniformly inside the cell suspension. We assessed cell proliferation, clonogenic survival and delayed luminescence of treated cells. Apoptosis and cell cycle distributions were measured by flow-cytometry. Irradiation with protons produced a modest increase in the apoptotic rate, but blocked the cell cycle at the G2/M phase for at least 48 h after irradiation, suggesting the presence of severe DNA damage. A 34% reduction of the DL quantum yield was observed in a specific DL time interval, 1-10 ms after the laser excitation of the cell samples after 1 h from irradiation, whereas the DL quantum yield exhibited an increase of 27% in the DL time interval 0.1-1 ms in cell samples probed 24 h after irradiation. The treatments using menadione, hydrogen peroxide and quercetin as oxidant agents potently induced apoptosis of Jurkat cells in a dose-dependent manner and consistently decreased the intensity of delayed photoemission. We obtained a strong anti-correlation between apoptosis of human leukemia Jurkat cells and UV-induced delayed photoemission on a specific time interval ranging from 100 μs to 1 ms after UV-excitation of the cell samples.
|Titolo:||Apoptosis, cell cycle and delayed luminescence of human leukemia Jurkat T-cells under proton-irradiation and oxidative stress conditions|
|Data di pubblicazione:||2010|
|Citazione:||Apoptosis, cell cycle and delayed luminescence of human leukemia Jurkat T-cells under proton-irradiation and oxidative stress conditions / I. BARAN; C. GANEA; A. SCORDINO; BARRESI V; F. MUSUMECI; S. TUDISCO; S. PRIVITERA; R. GRASSO; R. GRASSO; D.F. CONDORELLI; I. URSU; V. BARAN; E. KATONA; M.M. MOCANU; R. UNGUREANU; N. MUSSO; M. GULINO; G.A.P. CIRRONE; G. COTTONE; L.M. VALASTRO. - In: INFN NOTIZIE. - ISSN 1720-3287. - INFN-LNS- Activity Report 2009(2010), pp. 246-249.|
|Appare nelle tipologie:||1.1 Articolo in rivista|