We investigated the relation between apoptosis anddelayed luminescence (DL) in human leukemia Jurkat Tcellsundergoing various treatments. We used menadione, hydrogen peroxide and quercetin to induce oxidative stress conditions under different doses and treatment times. We irradiated Jurkat cells by using modulated beams of accelerated protons with energies up to 62 MeV, under a dose of 10 Gy distributed uniformly inside the cell suspension. We assessed cell proliferation, clonogenic survival and delayed luminescence of treatedcells. Apoptosis and cell cycle distributions weremeasured by flow-cytometry. Irradiation with protonsproduced a modest increase in the apoptotic rate, butblocked the cell cycle at the G2/M phase for at least 48 h after irradiation, suggesting the presence of severe DNA damage. A 34% reduction of the DL quantum yield was observed in a specific DL time interval, 1-10 ms after the laser excitation of the cell samples after 1 h fromirradiation, whereas the DL quantum yield exhibited anincrease of 27% in the DL time interval 0.1-1 ms in cell samples probed 24 h after irradiation. The treatments using menadione, hydrogen peroxide and quercetin as oxidant agents potently induced apoptosis of Jurkat cells in a dose-dependent manner and consistently decreasedthe intensity of delayed photoemission. We obtained astrong anti-correlation between apoptosis of humanleukemia Jurkat cells and UV-induced delayed photoemission on a specific time interval ranging from100 μs to 1 ms after UV-excitation of the cell samples.

Apoptosis, cell cycle and delayed luminescence of human leukemia Jurkat T-cells under proton-irradiation and oxidative stress conditions

SCORDINO, Agata;BARRESI, VINCENZA;MUSUMECI, Francesco;GRASSO, ROSARIA;CONDORELLI, Daniele Filippo;
2010

Abstract

We investigated the relation between apoptosis anddelayed luminescence (DL) in human leukemia Jurkat Tcellsundergoing various treatments. We used menadione, hydrogen peroxide and quercetin to induce oxidative stress conditions under different doses and treatment times. We irradiated Jurkat cells by using modulated beams of accelerated protons with energies up to 62 MeV, under a dose of 10 Gy distributed uniformly inside the cell suspension. We assessed cell proliferation, clonogenic survival and delayed luminescence of treatedcells. Apoptosis and cell cycle distributions weremeasured by flow-cytometry. Irradiation with protonsproduced a modest increase in the apoptotic rate, butblocked the cell cycle at the G2/M phase for at least 48 h after irradiation, suggesting the presence of severe DNA damage. A 34% reduction of the DL quantum yield was observed in a specific DL time interval, 1-10 ms after the laser excitation of the cell samples after 1 h fromirradiation, whereas the DL quantum yield exhibited anincrease of 27% in the DL time interval 0.1-1 ms in cell samples probed 24 h after irradiation. The treatments using menadione, hydrogen peroxide and quercetin as oxidant agents potently induced apoptosis of Jurkat cells in a dose-dependent manner and consistently decreasedthe intensity of delayed photoemission. We obtained astrong anti-correlation between apoptosis of humanleukemia Jurkat cells and UV-induced delayed photoemission on a specific time interval ranging from100 μs to 1 ms after UV-excitation of the cell samples.
delayed luminescence; apoptosis; Proton Irradiation
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/52871
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