Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coat- ings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the eluci- dation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylen- imine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompati- bility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell–substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity.
Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle
Anna AlbaWriting – Original Draft Preparation
;Giusy VillaggioData Curation
;Grazia Maria Lucia MessinaInvestigation
;Massimo CarusoInvestigation
;Concetta FedericoInvestigation
;Maria Teresa CambriaInvestigation
;Giovanni Marletta
Methodology
;Fulvia Sinatra
Conceptualization
2022-01-01
Abstract
Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coat- ings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the eluci- dation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylen- imine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompati- bility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell–substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity.File | Dimensione | Formato | |
---|---|---|---|
polymers-14-02643.pdf
accesso aperto
Descrizione: PDF
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
4.09 MB
Formato
Adobe PDF
|
4.09 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.