The species identity on anchovy products was tested through COI mitochondrial DNA sequences analysisin 50 samples belonging to 20 different commercial lots, such as anchovy fillets in vegetal oil, cannedanchovies and anchovy paste. Seven samples (14%) were found to be not from Engraulis encrasicolusmeats, confirming concerns of species substitution. Conventional COI-DNA barcoding revealed thepresence of four different species, E. encrasicolus, Engraulis japonicus, Sardinella aurita and Sardina pilchardus,in the processed products labeled as European anchovy. The DNA barcoding was then used incombination with PCR-RFLP method to investigate labeling accuracy in processed anchovy products andto unveil putative fish fraud involving the replacement of the European anchovy, E. encrasicolus, with lessvaluable Engraulidae and Clupeidae species. We applied a COIBar-RFLP (Cytochrome Oxidase I Barcode-Restriction Fragment Length Polymorphism) analysis that yielded differential patterns allowing theunambiguous discrimination of European anchovy from the other species tested. The proposed molecularstrategy relies on the efficiency of COI as a DNA barcode and proved very efficient and less expensivethan DNA sequencing strategies. This approach may be useful in routine controls, especially in cases oflarge-scale sample screening.

A COIBar-RFLP strategy for the rapid detection of Engraulis encrasicolus in processed anchovy products

PAPPALARDO AM;FERRITO, Venera
2015-01-01

Abstract

The species identity on anchovy products was tested through COI mitochondrial DNA sequences analysisin 50 samples belonging to 20 different commercial lots, such as anchovy fillets in vegetal oil, cannedanchovies and anchovy paste. Seven samples (14%) were found to be not from Engraulis encrasicolusmeats, confirming concerns of species substitution. Conventional COI-DNA barcoding revealed thepresence of four different species, E. encrasicolus, Engraulis japonicus, Sardinella aurita and Sardina pilchardus,in the processed products labeled as European anchovy. The DNA barcoding was then used incombination with PCR-RFLP method to investigate labeling accuracy in processed anchovy products andto unveil putative fish fraud involving the replacement of the European anchovy, E. encrasicolus, with lessvaluable Engraulidae and Clupeidae species. We applied a COIBar-RFLP (Cytochrome Oxidase I Barcode-Restriction Fragment Length Polymorphism) analysis that yielded differential patterns allowing theunambiguous discrimination of European anchovy from the other species tested. The proposed molecularstrategy relies on the efficiency of COI as a DNA barcode and proved very efficient and less expensivethan DNA sequencing strategies. This approach may be useful in routine controls, especially in cases oflarge-scale sample screening.
2015
COIBar-RFLP; European anchovy; Food traceability
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/55425
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