Neurospheres from the subventricular zone of adult mice were used as an experimental model to analyse the early differential effects of 17 beta-estradiol (17 beta-E2). Both floating and differentiating neurospheres expressed estrogen receptors (ERs) alpha and beta. The initial phases of differentiation coincided with a peak of ER alpha expression as by Western blot analysis. Treatment with 10 nM 17 beta-E2 induced a significant increase in the glial fibrillary acidic protein (GFAP)-positive population and a greater expression of GFAP, an effect sensitive to the estrogen receptor antagonist ICI 182,780. The GFAP-positive cell population induced by 17 beta-E2 was characterized by a highly differentiated phenotype and intense inummostaining as by immunocytochemistry and flow cytometry. These cells co-expressed ER alpha and were positive to BrdU. 17 beta-E2 also affected neuronal differentiation by rapidly and transiently increasing the percentage of polysialylated-neural cell adhesion molecule (PSA-NCAM) -positive progenitors, and by accelerating the appearance of a mature neuronal phenotype, as evaluated by micrombule-associated protein 2 (MAP2) staining. Our results point to a key role for ER alpha during initial phases of differentiation of brain cells and to an effect of 17 beta-E2 that sequentially involves both glia and neurons
Astrocyte-like cells as a main target for estrogen action during neuronal differentiation
MERLO S;VANCHERI, CARLO;COPANI, Agata Graziella;SORTINO, Maria Angela
2007-01-01
Abstract
Neurospheres from the subventricular zone of adult mice were used as an experimental model to analyse the early differential effects of 17 beta-estradiol (17 beta-E2). Both floating and differentiating neurospheres expressed estrogen receptors (ERs) alpha and beta. The initial phases of differentiation coincided with a peak of ER alpha expression as by Western blot analysis. Treatment with 10 nM 17 beta-E2 induced a significant increase in the glial fibrillary acidic protein (GFAP)-positive population and a greater expression of GFAP, an effect sensitive to the estrogen receptor antagonist ICI 182,780. The GFAP-positive cell population induced by 17 beta-E2 was characterized by a highly differentiated phenotype and intense inummostaining as by immunocytochemistry and flow cytometry. These cells co-expressed ER alpha and were positive to BrdU. 17 beta-E2 also affected neuronal differentiation by rapidly and transiently increasing the percentage of polysialylated-neural cell adhesion molecule (PSA-NCAM) -positive progenitors, and by accelerating the appearance of a mature neuronal phenotype, as evaluated by micrombule-associated protein 2 (MAP2) staining. Our results point to a key role for ER alpha during initial phases of differentiation of brain cells and to an effect of 17 beta-E2 that sequentially involves both glia and neuronsFile | Dimensione | Formato | |
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