Lipid vesicles made up of dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were used as a biological membrane model to investigate the interaction between natural and modified β-cyclodextrins and these membrane bilayers. Differential scanning calorimetry was used to study the thermotropic behavior of the DPPC vesicles and any change caused by the presence of cyclodextrins. The presence of dimethyl-β-cyclodextrin (DM-β-CyD) triggered a reduction in the enthalpy values related to the main transition peak from gel state to liquid crystal phase of DPPC aqueous dispersions, as a function of the DM-β-CyD molar fraction: the larger the amount of DM-β-CyD, the greater the reduction in ΔHvalues. This effect was probably due to the ability of DM-β-CyD to extract and to complex the DPPC molecules forming the phospholipid vesicles. The presence of β-cyclodextrin (β-CyD) or hydroxypropyl-β-cyclodextrin (HP-β-CyD) caused no particular alteration in the thermotropic parameters of DPPC vesicles, whereas trimethyl-β-cyclodextrin (TM-β-CyD) at molar fractions higher than 0.12 caused broadening of the transition peak due to a possible interaction with the hydrophobic part of the bilayers. Experiments on DPPC–cholesterol (10 mol%) vesicles showed the capability of β-CyD and TM-β-CyD to extract cholesterol from the ordered bilayer structures, triggering an alteration in the lipid constituents of the membranes. HP-β-CyD caused no variation in the thermotropic parameters of the DPPC–cholesterol (10 mol%) vesicles. The findings show that HP-β-CyD seems the most suitable molecular drug carrier forin vivoadministration.
Interaction of Natural and Modified β-Cyclodextrins with a Biological Membrane Model of Dipalmitoylphosphatidylcholine
PUGLISI, Giovanni;
1996-01-01
Abstract
Lipid vesicles made up of dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were used as a biological membrane model to investigate the interaction between natural and modified β-cyclodextrins and these membrane bilayers. Differential scanning calorimetry was used to study the thermotropic behavior of the DPPC vesicles and any change caused by the presence of cyclodextrins. The presence of dimethyl-β-cyclodextrin (DM-β-CyD) triggered a reduction in the enthalpy values related to the main transition peak from gel state to liquid crystal phase of DPPC aqueous dispersions, as a function of the DM-β-CyD molar fraction: the larger the amount of DM-β-CyD, the greater the reduction in ΔHvalues. This effect was probably due to the ability of DM-β-CyD to extract and to complex the DPPC molecules forming the phospholipid vesicles. The presence of β-cyclodextrin (β-CyD) or hydroxypropyl-β-cyclodextrin (HP-β-CyD) caused no particular alteration in the thermotropic parameters of DPPC vesicles, whereas trimethyl-β-cyclodextrin (TM-β-CyD) at molar fractions higher than 0.12 caused broadening of the transition peak due to a possible interaction with the hydrophobic part of the bilayers. Experiments on DPPC–cholesterol (10 mol%) vesicles showed the capability of β-CyD and TM-β-CyD to extract cholesterol from the ordered bilayer structures, triggering an alteration in the lipid constituents of the membranes. HP-β-CyD caused no variation in the thermotropic parameters of the DPPC–cholesterol (10 mol%) vesicles. The findings show that HP-β-CyD seems the most suitable molecular drug carrier forin vivoadministration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
