Investigation of the Self Incompatibility Mechanism in Clementine (Citrus clementina Hort. ex Tan.) Using cDNA-AFLP

are involved in the self-incompatibility mechanism of Clementine mandarins.Pollen tube growth of clementine ‘Algerian Tangerine’ (self-incompatible) wasanalyzed using the complementary DNA-amplified fragment length polymorphismtechnique to identify genes putatively involved in self-incompatibility. The transcriptprofiles of unpollinated and self-pollinated (for 1, 2, 3, 4, 5, 6, 7th days) styles andstigmas of ‘Algerian Tangerine’ were compared. Complementary DNA profilesshowing transcriptional differences between unpollinated and self-pollinated stigmasand styles were excised from the gels and directly sequenced. Some unigenes weredetermined from the results of direct cDNA sequencing. Selected gene tags showedtranscriptional differences between unpollinated and self-pollinated styles and stigmasduring pollen germination and pollen tube elongation. The differential expression ofsome sequences was confirmed with the quantitative reverse transcription-polymerase(RT PCR) chain reaction.