The proteins belonging to the cereal trypsin/alpha-amylase inhibitor family are abundant water/salt-soluble flour proteins active against alpha-amylases from several seed parasites and pests and inactive against enclogenous alpha-amylases. Three alpha-amylase inhibitor families have been described in cereals that vary in size and are differently expressed among Triticeae seeds. The present work investigates the presence of human salivary alpha-amylase inhibitors in emmer (Triticum dicoccon Schrank) flour. The isolation was obtained by a series of chromatography steps, and the purification progress was monitored through the inhibition of human salivary alpha-amylase activity. The purified fraction was subjected to protein sequencing by tandem mass spectrometry (MSMS) of the tryptic digests obtained after the sample separation on 2-DE. MSMS data indicated that the emmer alpha-amylase inhibitory fraction was composed of two newly identified proteins [emmer dimeric inhibitor 1 (EDI-1) and emmer dimeric inhibitor 2 (EDI-2)] sharing very high identity levels with related proteins from Triticum aestivum.
|Titolo:||Dimeric inhibitors of human salivary alfa-amylase from emmer (Triticum dicoccon Schrank) seeds|
|Data di pubblicazione:||2007|
|Citazione:||Dimeric inhibitors of human salivary alfa-amylase from emmer (Triticum dicoccon Schrank) seeds / FONTANINI D; CAPOCCHI A; MUCCILLI V; SAVIOZZI F; CUNSOLO V; SALETTI R; FOTI S; GALLESCHI L. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 55:25(2007), pp. 10452-10460.|
|Appare nelle tipologie:||1.1 Articolo in rivista|