The practical and easy detection of dopamine levels in human fluids, such as urine and saliva, is of great interest due to the correlation of dopamine concentration with several diseases. In this work, the one-step synthesis of water-soluble carbon nanoparticles (CNPs), starting from artichoke extract, containing catechol groups, for the fluorescence sensing of dopamine is reported. Size, morphology, chemical composition and electronic structure of CNPs were elucidated by DLS, AFM, XPS, FT-IR, EDX and TEM analyses. Their optical properties were then explored by UV-vis and fluorescence measurements in water. The dopamine recognition properties of these CNPs were investigated in water through fluorescence measurements and we observed the progressive enhancement of the CNP emission intensity upon the progressive addition of dopamine, with a binding affinity value of log K = 5.76 and a detection limit of 0.81 nM. Selectivity towards dopamine was tested over other interfering analytes commonly present in human saliva. Finally, in order to perform a solid point of care test, CNPs were adsorbed on a solid support and exposed to different concentrations of dopamine, thus observing a pseudo-linear response, using a smartphone as a detector. Therefore, the detection of dopamine in simulated human saliva was performed with excellent results, in terms of selectivity and a detection limit of 100 pM.
Dopamine sensing by fluorescent carbon nanoparticles synthesized using artichoke extract
Roberta Puglisi;Rossella Santonocito;Antonino Gulino;Valentina Oliveri;Roberta Ruffino;Giovanni Li Destri;Vera Muccilli;Nunzio Cardullo;Nunzio Tuccitto;Andrea Pappalardo;Giuseppe Trusso Sfrazzetto
2024-01-01
Abstract
The practical and easy detection of dopamine levels in human fluids, such as urine and saliva, is of great interest due to the correlation of dopamine concentration with several diseases. In this work, the one-step synthesis of water-soluble carbon nanoparticles (CNPs), starting from artichoke extract, containing catechol groups, for the fluorescence sensing of dopamine is reported. Size, morphology, chemical composition and electronic structure of CNPs were elucidated by DLS, AFM, XPS, FT-IR, EDX and TEM analyses. Their optical properties were then explored by UV-vis and fluorescence measurements in water. The dopamine recognition properties of these CNPs were investigated in water through fluorescence measurements and we observed the progressive enhancement of the CNP emission intensity upon the progressive addition of dopamine, with a binding affinity value of log K = 5.76 and a detection limit of 0.81 nM. Selectivity towards dopamine was tested over other interfering analytes commonly present in human saliva. Finally, in order to perform a solid point of care test, CNPs were adsorbed on a solid support and exposed to different concentrations of dopamine, thus observing a pseudo-linear response, using a smartphone as a detector. Therefore, the detection of dopamine in simulated human saliva was performed with excellent results, in terms of selectivity and a detection limit of 100 pM.File | Dimensione | Formato | |
---|---|---|---|
Dopamine sensing by fluorescent carbon nanoparticles synthesized using artichoke extract.pdf
accesso aperto
Descrizione: Articolo
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
1.73 MB
Formato
Adobe PDF
|
1.73 MB | Adobe PDF | Visualizza/Apri |
Supporting Information revised no highlighted.pdf
accesso aperto
Descrizione: Supporting Information
Tipologia:
Altro materiale allegato
Licenza:
Creative commons
Dimensione
942.46 kB
Formato
Adobe PDF
|
942.46 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.