A low molecular weight (MW) lectin (Paracentrotus lividus small lectin, PlSL) has been found in the sea urchin, Paracentrotus lividus. After gel electrophoresis under denaturing conditions, PlSL exhibits a MW of 13 kDa, while its hemagglutinating activity is Ca2+-independent and inhibited by D-Glucose, L-Rhamnose, D-Arabinose, L-Fucose and N-Acetyl-D-glucosamine. Electrophoretic analysis of the coleomic fluid of P. lividus reveals that the presence of PlSL increases following immune challenge with bacteria, whereas it is annulled as a consequence of osmotic stress. Interestingly, two other putative inducible hemagglutinins of an approximate MW of 11 and 32 kDa were retrieved in concomitance with the stress-promoted disappearance of PlSL.

Presence of a low molecular weight lectin in the coelomic fluid of the sea urchin Paracentrotus lividus

PEZZINO, FRANCA MARIA;D'URSO, Vera;
2009

Abstract

A low molecular weight (MW) lectin (Paracentrotus lividus small lectin, PlSL) has been found in the sea urchin, Paracentrotus lividus. After gel electrophoresis under denaturing conditions, PlSL exhibits a MW of 13 kDa, while its hemagglutinating activity is Ca2+-independent and inhibited by D-Glucose, L-Rhamnose, D-Arabinose, L-Fucose and N-Acetyl-D-glucosamine. Electrophoretic analysis of the coleomic fluid of P. lividus reveals that the presence of PlSL increases following immune challenge with bacteria, whereas it is annulled as a consequence of osmotic stress. Interestingly, two other putative inducible hemagglutinins of an approximate MW of 11 and 32 kDa were retrieved in concomitance with the stress-promoted disappearance of PlSL.
Paracentrotus lividus, sea urchin, lectin, immunity, stress
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/6708
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