The aim of the present investigation was to study the effects of choline and cholinecontaining phospholipids CDP-choline (CDPC) and L-alpha-glyceryl-phosphorylcholine (alpha-GPC) on transglutaminase (TG) activity and expression in primary astrocyte cultures. TG is an important Ca2+ -dependent protein that represents a normal constituent of nervous systems during fetal stages of development, playing a role in cell signal transduction, differentiation, and apoptosis. Confocal laser scanning microscopy (CLSM) analysis showed an increase of TG activity in astrocyte cultures treated with choline, CDPC, or alpha-GPC at 0.1 mM or 1 mM concentrations. Comparatively, alpha-GPC induced the most conspicuous effects enhancing monodansyl-cadaverine fluorescence both in cytosol and in nuclei, supporting the evidence of the important role played by alpha-GPC throughout differentiation processes tightly correlated to nucleus-cytosol cross-talk during astroglial cells proliferation and development. Western blot analysis showed that in 24h 1 mM alpha-GPC and choline-treated astrocytes increased TG-2, whereas no effect was observed in 24h 1 mM CDP-choline treated astrocytes. Our data suggest a crucial role of choline precursors during different stages of astroglial cell proliferation and differentiation in cultures.
|Titolo:||Effect of Choline-Containing Phospholipids on Transglutaminase Activity in Primary Astroglial Cell Cultures|
|Data di pubblicazione:||2008|
|Citazione:||Effect of Choline-Containing Phospholipids on Transglutaminase Activity in Primary Astroglial Cell Cultures / BRAMANTI V; BRONZI D; TOMASSONI D; LI VOLTI G; CANNAVO G; RACITI G; NAPOLI M; VANELLA A; CAMPISI A; IENTILE R; AVOLA R. - In: CLINICAL AND EXPERIMENTAL HYPERTENSION. - ISSN 1064-1963. - 30(2008), pp. 798-807.|
|Appare nelle tipologie:||1.1 Articolo in rivista|