Comparative analysis of storage compound metabolism in the embryo and endosperm of Cakile maritima seeds

Seed production plays an essential role in the life cycle of angiosperms. Within the Brassicaceae family, seed maturation has been extensively studied in the model plant Arabidopsis and several agronomically important species. In this study, we investigate this developmental process in Cakile maritima, a wild halophytic member of the Brassicaceae that thrives along sandy coastlines from the Arctic to the Mediterranean. By independently analysing the different zygotic tissues of the seed, namely, the embryo and the endosperm, we demonstrate that, quantitatively, the embryo is the main site of storage compound accumulation. Notably, the biochemical composition of these reserves differs substantially between the zygotic tissues. Approximately half of the fatty acids in the endosperm consist of omega-7 monounsaturated fatty acids, which are largely absent in the embryo. By contrast, the embryo shows a stronger induction of omega-9 and polyunsaturated fatty acid biosynthesis pathways compared with the endosperm, reflecting tissue-specific regulation of fatty acid metabolism genes. Furthermore, seeds collected from different ecological niches along a latitudinal gradient reveal that environmental temperature significantly affects the composition of seed reserves, particularly the proportion of polyunsaturated fatty acids in the embryo. Together, these findings underscore the metabolic diversity and adaptative potential of C. maritima.In Cakile maritima seeds, the storage compounds are unevenly distributed between the embryo and the endosperm, with differing compositions that can be influenced by environmental conditions such as temperature.