In the study of the uterine cervix carcinoma the virus HPV, considered as etiological agents, have a real importance. Screening programs by cervical cytology (Pap test or Papanicolau test) have caused a drastic reduction in the incidence cervix carcinoma. This was particularly due to the simplicity of the sampling and to the low cost of the method; it gives the possibility of working on large number of specimens in a short period of time. Nevertheless this test has both a limited sensibility and peculiarity, especially when there is a low grade injury. The use of the molecular biology in the study both of pre-cancerous injuries and cancerous ones gives the opportunity for a more careful diagnosis of HPV infection. In this study genotyping for HPV was carried out in biopsies taken from patients affected from carcinoma in situ or invasive carcinoma. The results obtained confirm the predominant role of HPV 16 as it described in literature; but in our geographic area, the genotype 18 has not as much relevance as it has been stated in other nations. Moreover, the use of samples of paraffin wax embedded tissues offers the opportunity of doing retrospective researches that can give the opportunity of a deeper knowledge of the epidemiological course of the HPV and its role in cervix carcinoma.
Nello studio del carcinoma della cervice uterina molta rilevanza assumono i papillomavirus umani quali agenti eziologici. Programmi di screening mediante citologia cervicale (Pap test o test di Papanicolau) hanno consentito una drastica riduzione dell’incidenza del cancro alla cervice, grazie soprattutto alla semplicità di prelievo ed al basso costo dell’indagine che consente di lavorare su grandi numeri ed in tempi brevi; tuttavia questo test ha una sensibilità e una specificità limitata, specialmente in casi di basso grado di lesione (low grade). L’uso della biologia molecolare nello studio delle lesioni neoplastiche di vario grado fornisce l’opportunità per una più accurata diagnosi di infezione da HPV. In questo studio sono stati eseguiti test di genotipizzazione in campioni bioptici ottenuti da pazienti affette da carcinoma in situ o carcinoma invasivo. I risultati confermano il ruolo predominante di HPV 16 così come descritto in letteratura internazionale ma appare evidente che il genotipo 18 nella nostra area geografica non ha la rilevanza che viene descritta in altre nazioni. L’impiego di campioni di tessuto fissati e paraffinati, inoltre, offre l’opportunità di compiere indagini retrospettive che possono consentire una più approfondita conoscenza dell’andamento epidemiologico di HPV e del suo effettivo ruolo nella genesi del carcinoma della cervice uterina.
Use of paraffin wax embedded biopsies from cervix carcinoma for HPV genotyping [Impiego di campioni paraffinati di lesioni cancerose della cervice uterina nella genotipizzazione di HPV]
RUSSO, Raffaela;SCALIA, Guido
2010-01-01
Abstract
In the study of the uterine cervix carcinoma the virus HPV, considered as etiological agents, have a real importance. Screening programs by cervical cytology (Pap test or Papanicolau test) have caused a drastic reduction in the incidence cervix carcinoma. This was particularly due to the simplicity of the sampling and to the low cost of the method; it gives the possibility of working on large number of specimens in a short period of time. Nevertheless this test has both a limited sensibility and peculiarity, especially when there is a low grade injury. The use of the molecular biology in the study both of pre-cancerous injuries and cancerous ones gives the opportunity for a more careful diagnosis of HPV infection. In this study genotyping for HPV was carried out in biopsies taken from patients affected from carcinoma in situ or invasive carcinoma. The results obtained confirm the predominant role of HPV 16 as it described in literature; but in our geographic area, the genotype 18 has not as much relevance as it has been stated in other nations. Moreover, the use of samples of paraffin wax embedded tissues offers the opportunity of doing retrospective researches that can give the opportunity of a deeper knowledge of the epidemiological course of the HPV and its role in cervix carcinoma.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.