ISOLAMENTO E CARATTERIZZAZIONE DI STREPTOCOCCUS AGALACTIAE IN DONNE GRAVIDE

During the past several decades Streptococcus agalactiae has emerged as the major cause of bacterial infection in newborns with a high rate of mortality. In the present study the maternal colonisation, the distribution of serotypes, the efficacy of intrapartum antibiotic-prophylaxis and the prevalence of same factor of virulence have been estimated. Particularly on GBS isolates a study has been made to identify the genes of surface proteins "alp", biofilm formation, antimicrobial susceptibility and macrolide resistence genotypes. A total of 60 women at 35th-37th week of gestation were enrolled, to these, during the screening for prevention of perinatal GBS disease, double samples have been made, from the vagina and the rectum. About 12% of the pregnant women was colonised by GBS and the characterized serotypes were: Ia, III and V. About 72% of the isolated GBS express "alp" proteins. The greater biofilm formation is obtained in THB with 1% of glucose and in atmosphere containing 5% CO2. All isolated GBS have been susceptible to ampicillin but about 28,5% have been resistent to the other tested antibiotics (macrolide and lincosamides). All colonisated women were undergone to intrapartum antibioticprophylaxis and physiological course of puerperium and especially the lack of events in children of women undergoing treatment demonstrates the effectiveness of prophylaxis.