Amyloid peptide (A), generated by proteolytic cleavage of the amyloid precursor protein (APP), plays a pivotal role in the pathogenesis of Alzheimer's disease (AD). The key step in the generation of A is cleavage of APP by -secretases (beta-site APP-cleaving enzyme 1 (BACE1) and BACE2). There has been suggestion of interaction between aluminum and several AD-associated pathways. However, the underlying mechanisms still remain unclear. Here, we report the effects of aluminum chloride (AlCl(3)) in A-induced toxicity using differentiated neuronal SH-SY5Y cells. The metal significantly enhances A-induced cell death at concentrations ranging from 50 to 300 A mu M after 24 and 48 h. After 72 and 96 h treatment, cell death is increased already at 10 A mu M. Early coexposure of cells to 10 A mu M AlCl(3) and 2 A mu M A differentially affected -secretase mRNA levels as compared to single A treatment after 1 and 3 h. BACE1 levels were slightly reduced after 1 h and significantly increased after 3 h exposure, whereas BACE2 levels were increased at both times considered. Both genes' mRNA levels were downregulated at longer times (6, 12, and 24 h). Although these results indicate that aluminum toxicity is correlated to changes in both BACE1 and BACE2 expression levels, the subsequent common downregulation observed suggests that aluminum involvement in the A cascade is subtle, and other underlying mechanisms might be involved.
|Titolo:||Early effects of aluminium chloride on beta-secretase mRNA expression in a neuronal model of beta-amyloid toxicity|
|Autori interni:||D'AGATA, VELIA MARIA|
|Data di pubblicazione:||2010|
|Rivista:||CELL BIOLOGY AND TOXICOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|