In the course of our continuing search for new natural anticancer compounds for treatment and/or prevention of prostate cancer, our laboratory has focused its search on poorly investigated lichen metabolites, sphaerophorin, pannarin and epiphorellic acid-1. To this end, we treated DU-145, a cell line resembling the last stage of prostate carcinoma, with different concentrations (6-50 μmol/l) of these compounds for 72 h. Our data clearly evidenced that these lichen metabolites inhibit the growth of human prostate carcinoma DU-145 cells, but pannarin exhibits a higher effect. Our data show an induction of apoptotic death of advanced prostate cancer cells by sphaerophorin, pannarin and epiphorellic acid-1. In fact, a significant (P<0.001) increase in caspase-3 enzyme activity occurred in DU-145 cells treated with all lichen compounds at 12 and 25 μmol/l concentrations, correlated to a high DNA fragmentation, but without the disruption of the plasma membrane, as evaluated by the percentage of lactic dehydrogenase release. Alternatively, we found a low, but significant (P<0.01) lactic dehydrogenase release at higher concentrations (50 μmol/l), suggesting that in these experimental conditions sphaerophorin, pannarin and epiphorellic acid-1 induce necrosis in DU-145 cells, through the increase in reactive oxygen species generation. The experimental evidence is further confirmed by caspase-3 activity results, evidencing a reduction in the activity of this protease at a higher concentration, 50 μmol/l.
Titolo: | Pannarin inhibits cell growth and induces cell death in human prostate carcinoma DU-145 cells |
Autori interni: | |
Data di pubblicazione: | 2006 |
Rivista: | |
Citazione: | Pannarin inhibits cell growth and induces cell death in human prostate carcinoma DU-145 cells / Russo, Alessandra; Piovano, M; L, Lombardo; Vanella, Luca; Cardile, Venera; Garbarino, J.. - In: ANTI-CANCER DRUGS. - ISSN 0959-4973. - 17:10(2006), pp. 1163-1169. |
Handle: | http://hdl.handle.net/20.500.11769/8341 |
Appare nelle tipologie: | 1.1 Articolo in rivista |