ANTIOXIDANT AND PROTECTIVE EFFECTS OF SUMAC LEAVES ON CHONDROCYTES

In this study we evaluate in vitro antioxidant and chondroprotective effects of a lyophilised hydroalcholic extract from Sumac leaves. The extract contains many constituents as flavones, tannins, anthocyanins and organic acids with several known biological effects. The antioxidant activity of extract was assayed employing 1, 1-diphenyl-2-picrylhydrazyl radical scavenging method (DPPH). The antioxidative/chondroprotective effect was evaluated on the production of key molecules released during degenerative osteoarthritis disease (OA) such as nitric oxide (NO), reactive oxygen species (ROS), glycosaminoglycans (GAGs), and prostaglandins (PGE2) in human chondrocyte cultures, stimulated with pro-inflammatory cytokine interleukin-1 (IL-1). As result, the extract from Sumac leaves was effective in reducing the stable free radical DPPH, and in vitro was able to contrast the harmful effects of IL-1 beta.