Detection of glucosinolate metabolite pathway using SSR markers of Brassica oleracea complex species (n=9) core collection

The use of genetic approaches to analyse the genetic variation associated with morphological and biochemical differences has greatly facilitated the improvement of agronomic traits. In this context, the aim of this research was to associate glucosinolates (GLS) profile of Brassica oleracea complex species (n=9) core collection with SSR markers. In this study, we have analysed several accessions belonging to 4 landraces, 5 wild species and 4 hybrids F1 Brassica crops from different origin. The 12 SSR markers involved allowed the identification of highly informative primer combination and highlighted significant patterns of variation among the accessions. The content of glucosinolates was determined using the HPLC diode array detector (DAD) technique using different standards. The results showed a great amount of variation, qualitatively and quantitavely, between and among Brassica accessions. The major glucosinolate detected in all accessions was glucoraphanin GRA. The highest GLS content was observed in the wild species Brassica rupesteris with a value of 46,37 µmoles g-1 dry weight. A significant correlation, between GLS and SSR markers linked to genes involved in the biosynthesis of aliphatic, aromatic and indolic glucosinolate content, was observed. Our results suggest the presence of a large variability among the Brassica oleracea complex species analysed, that could be useful for organic breeding of the GLS profiles as required.