Field flow fractionation dielectrophoretic (FFF-DEP) devices are currently used, among the others, for the separation of tumor cells from healthy blood cells. To this end specific suspension/elution buffers (EBs), with reduced conductivity (with respect to that of the cell cytoplasm) are generally used. In this paper we investigate the long-term alterations of the cells and elution buffers. We find that the EB conductivity is critically modified within few minutes after cells suspension. In turn, this modification results in a change the ideal separation frequency of the FFF-DEP device. On the other hand we prove that DEP manipulation is preserved for more than three hours for cells suspended in the considered EBs.

Analysis of the role of elution buffers on the separation capabilities of dielectrophoretic devices

Rossana Di Martino;Massimo Camarda;Michele Cascio;Salvatore Baldo;Alessandra Romano;Luigi Minafra;Francesco Di Raimondo;Silvia Scalese;Antonino La Magna
2016-01-01

Abstract

Field flow fractionation dielectrophoretic (FFF-DEP) devices are currently used, among the others, for the separation of tumor cells from healthy blood cells. To this end specific suspension/elution buffers (EBs), with reduced conductivity (with respect to that of the cell cytoplasm) are generally used. In this paper we investigate the long-term alterations of the cells and elution buffers. We find that the EB conductivity is critically modified within few minutes after cells suspension. In turn, this modification results in a change the ideal separation frequency of the FFF-DEP device. On the other hand we prove that DEP manipulation is preserved for more than three hours for cells suspended in the considered EBs.
2016
Cell motility; Circulating tumor cells; Dielectrophoresis; Elution buffer
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/628095
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